Assessment & comparison of hepatoprotective potential of indigenous therapeutic flora by using tetrachloromethane encouraged liver toxicity in Albino (Wister strain) rodent model.

Introduction: Indigenous herbs such as Momordica charantia L., Nardostachys jatamansi (D. Don) DC., Justicia adhatoda L. and Tephrosia purpurea (L.) Pers. are known to improve several disease characteristics including hepatoprotective activities. Objective: We aimed to demonstrate the hepatoprotective potential of indigenous herbs Momordica charantia L., Tephrosia purpurea(L.) Pers., Justicia adhatoda L. & Nardostachys jatamansi (D. Don) DC. in tetrachloromethane (CCl4) encouraged liver toxicity in Albino (Wister strain) rodent model. Methods: In Albino rats (Wister strain, n=114), hepatotoxicity was induced using CCl4 & the liver enzyme & histopathological profiles with an extent of the regenerative changes were evaluated. A total of 19 groups of rats were created containing six rats in each group & received the following - group 1: control group, group 2: CCl40.7 ml/kg, group 3: Liv-52 1 ml/kg. Light petroleum, trichloromethane, alcohol & watery extricates of Justicia adhatoda L.(200mg/kg), Tephrosia purpurea (L.) Pers. (200mg/kg), Momordica charantia L.(200mg/kg), & Nardostachys jatamansi (D. Don) DC.(500mg/kg)were administered in one group each (total 16 groups). Results-The trichloromethane extricate of Justicia adhatoda L, Light petroleum extricate of Nardostachys jatamansi (D. Don) DC. & Momordica charantia L., alcoholic extricates of Nardostachys jatamansi (D. Don) DC. displayed a noticeable reduction in the concentration of serum glutamic pyruvic transaminase SGPT), serum alkaline phosphatase (SALP), serum glutamic oxaloacetic transaminase (SGOT), bilirubin & other serum markers, indicating prominent hepatocytes with more regenerative activity, which signifies better hepatoprotective action as evaluated with other groups. Light petroleum & watery extricates of Justicia adhatoda L., Momordica charantia L. alcoholic extricates, Light petroleum, alcoholalcohol & trichloromethane extricates of Tephrosia purpurea (L.) Pers. showed moderate reductions in the liver enzyme levels & moderate degree of fatty changes. However, the alcoholic extricates of Justicia adhatoda L., & trichloromethane, watery extricate of Momordica charantia L., watery & trichloromethane extricate of Nardostachys jatamansi (D. Don) DC. & watery extricate of Tephrosia purpurea (L.) Pers. showed no reduction in the enzyme levels, indicating no activity. The total & direct bilirubin was also assessed in the blood serum, & there was no considerable difference among the groups. Conclusion: The hepatoprotective activity screening indicates that trichloromethane extricate of Justicia adhatoda L., Light petroleum extricate of Nardostachys jatamansi (D. Don) DC. & Momordica charantia L., showed more prominent hepatoprotective activity. Light petroleum & watery extricates of Justicia adhatoda L., alcoholic extricates of Nardostachys jatamansi (D. Don) DC. & Momordica charantia L., illustrated moderate antihepatotoxicity action, while alcoholic extricates of Justicia adhatoda L., trichloromethane & watery Gaurav Kumar Sharma et al/Assessment & comparison of hepatoprotective potential of indigenous therapeutic flora by using tetrachloromethane encouraged liver toxicity in Albino (Wister strain) rodent model 2603| International Journal of Pharmaceutical Research | Jan - Mar 2021 | Vol 13 | Issue 1 extricates of Momordica charantia L., watery & trichloromethane extricates of Nardostachys jatamansi (D. Don) DC., & none of the four extricates of Tephrosia purpurea (L.) Pers. showed hepatoprotective activities. [ABSTRACT FROM AUTHOR]