Evaluation of the cariogenic potential of a probiotic candidate strain Lactobacillus gasseri YIT 12321.

This study was designed to evaluate the cariogenic potential of Lactobacillus gasseri YIT 12321. L. gasseri YIT 12321 was isolated from the human oral cavity as a probiotic candidate strain. Three types of experimental biofilm formations on bovine enamel specimens were assessed using Streptococcus sobrinus ATCC 33478, L. gasseri YIT 12321 , and a co-culture of S. sobrinus and L. gasseri YIT 12321 in vitro. L. gasseri YIT 12321 was analyzed for its ability to utilize seven carbohydrates. L. gasseri YIT 12321 was cultured in tryptic soy broth without dextrose and containing a test carbohydrate at 37 °C for 16 h. The decrease in pH in the region under the biofilm produced by L. gasseri YIT 12321 was unusually slow, and the pH was maintained above 5.5 for 16 h. The amount of biofilm and the reduction in enamel hardness were minimal in the L. gasseri YIT 12321 group among the three experimental groups. The amount of co-culture-derived biofilms was less than that of S. sobrinus biofilms. When S. sobrinus was grown using sucrose, the pH decreased to 3.98. In contrast, L. gasseri YIT 12321 cultures maintained the pH above the critical pH for dentin demineralization. L. gasseri YIT 12321 is suggested to have a low cariogenic potential. The application of this strain as a probiotic may contribute to oral safety. • Lactobacillus gasseri YIT 12321 may have a low cariogenic potential as a probiotic. • L. gasseri YIT 12321 may have a low preference for the cariogenic carbohydrate sucrose. • The reduction in pH during growth of L. gasseri YIT 12321 was slow and minor. • L. gasseri YIT 12321 cultures contained a low content of water-insoluble glucan. [ABSTRACT FROM AUTHOR]