Photochemical protein modification in complex biological environments: recent advances and considerations for future chemical methods development.

As protein-protein interactions and other protein-interface interactions require molecular contact, high resolution spatiotemporal data is required in order to detect new proximal partners with high confidence. Chemistry for the analysis of protein-protein interactions: rapid and efficient cross-linking triggered by long wavelength light. In 1999, Kodadek reported two strategies for protein-protein cross-linking using either cationic palladium-porphyrins ( B 1 b ) ([12]) or ruthenium tris-bipyridyl complexes [Ru(II)(bpy) SB 3 sb ] SP 2+ sp ([8]) visible light, and (NH SB 4 sb ) SB 2 sb S SB 2 sb O SB 8 sb as a stoichiometric oxidant (Figure 2A). Keywords: bioconjugation; chemoproteomics; drug discovery; photochemistry EN bioconjugation chemoproteomics drug discovery photochemistry 413 420 8 03/11/22 20220301 NES 220301 Introduction Protein modification chemistry (commonly referred to as bioconjugation chemistry), when performed in complex biological mixtures, offers insight into cellular pathways and proteomes with remarkable precision. [Extracted from the article]