A signal amplification of near-infrared electrochemiluminescence immunosensor for SFTSV determination based on SiO2 photonic crystals nanomembrane.

In this work, a novel near-infrared electrochemiluminescence signal amplification strategy was fabricated based on SiO 2 photonic crystals nanomembrane as the ECL electrodes for sensitive and specific detection of SFTSV. The photonic crystal structure of the SiO 2 nanomembrane has an excellent light scattering effect, which significantly increases the photon flux in the luminescent molecule and improves the excitation of the ECL molecule (CdTe@CdS QDs) by the re-radiation dipole electromagnetic field. In addition, the increase of the effective electroactive area of the photonic crystals modified electrode also prominently contributes to the ECL intensity. The results manifested that the SiO 2 photonic crystals nanomembrane modified electrode constructed in this work can effectively improve the ECL intensity by about 7 times. Subsequently, a sandwich-type SFTSV immunosensor with a very low limit of detection of 0.0014 fg/mL was constructed. Due to the low cost and easy preparation of SiO 2 photonic crystal solid-state electrodes, this work can not only effectively detect the SFTSV virus, but also provides an effective ECL signal amplification strategy. • The ECL signal amplification mechanism of QDs based onphotonic crystals nanomembrane is firstly studied. • The NIR ECL immunosensor for SFTSV determination was firstly fabricated. • The enhanced NIR ECL immunosensor can sensitively detect SFTSV with an extremely low detection limit (0.0014 fg/mL). [ABSTRACT FROM AUTHOR]