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Discrepancy between sperm acrosin activity and sperm morphology: significance for fertilization in vitro
- Source :
-
Clinica Chimica Acta . Jan2005, Vol. 351 Issue 1/2, p121-129. 9p. - Publication Year :
- 2005
-
Abstract
- Abstract: Background: In routine semen analysis, discrepancies may occur between sperm acrosin activity test results and sperm acrosomal morphology. Methods: Discrepant test results of sperm acrosin activity (spectrophotometric assay) vs. sperm morphology (strict criteria) in the initial diagnostic investigation of 107 infertile couples were evaluated with respect to fertilization rate (% oocytes with 2 pronuclei) further obtained in IVF treatment. Results: Acrosin activity positively correlated with sperm morphology (% normal forms) (r=0.537) and fertilization rate (r=0.526). ROC curves for the prediction of ≥50% fertilization rate were comparable for acrosin activity and sperm morphology, with optimal cutoff values at 25 μIU/106 sperm and 10%, respectively. In multiple regression analysis, sperm acrosin activity (P=0.002) predicted fertilization rate independently of sperm morphology (P<0.001) and sperm vitality (eosin–nigrosin stain) (P=0.03). Acrosin activities ≥25 μIU/106 sperm were observed in 36% of severe teratozoospermic samples (≤4% normal spermatozoa) associated with low fertilization rate. Twenty percent of the morphologically normal ejaculates showed a low acrosin activity (<25 μIU/106 sperm) and low hypoosmotic swelling test (HOST) scores (31.4±7.6%) and were associated with low fertilization rate. Conclusion: The sperm acrosin assay can help to predict sperm fertilizing capacity in IVF independently of sperm morphology. [Copyright &y& Elsevier]
- Subjects :
- *SPERMATOZOA
*FERTILIZATION in vitro
*GENETIC engineering
*SEMEN
Subjects
Details
- Language :
- English
- ISSN :
- 00098981
- Volume :
- 351
- Issue :
- 1/2
- Database :
- Academic Search Index
- Journal :
- Clinica Chimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 15552439
- Full Text :
- https://doi.org/10.1016/j.cccn.2004.08.001