Screening of anti-tyrosinase and anti-bacterial activities of ethanolic extract from stink bean (Parkia speciosa Hassk) seed.

Research on tyrosinase inhibitors is attractive due to wide range of industrial application, such as in food, cosmetic and medicinal aspects. Synergistic effects on anti-tyrosinase and anti-bacterial activities can occur in natural products. Aims of this study were to screen anti-tyrosinase and anti-bacterial activities of ethanolic extract from stink bean seed (Parkia speciosa Hassk.) by dopachrome and disc diffusion methods, respectively. Fresh seeds were removed from pods and extracted with ethanol by maceration. Total phenolic content (TPC) measurement stink bean seed extract was done by Folin-Ciocalteu method. Anti-tyrosinase activity of extract were evaluated and compared with kojic acid. Anti-bacterial activity of extract against Propionibacterium acnes and Staphylococcus aureus was evaluated by disc diffusion method, and clindamycin (0.002 mg) and erythromycin (0.015 mg) were controlled as standard antibiotics, respectively. TPC of ethanolic extract from stink bean seed was 62.01±0.03 mg GAE/100 g of dried weight, however no in vitro inhibitory effect on tyrosinase enzyme when compared to kojic acid (IC50 = 0.01±0.0 mg/ml). Undiluted ethanol extract (8 mg) was inhibited P. acnes (inhibition zone = 7.30±0.35 mm); and was not inhibited S. aureus. Therefore, clindamycin (0.002 mg) and erythromycin (0.015 mg) were strongly inhibited P. acnes and S. aureus, respectively. This finding was first report for tyrosinase activity of stink bean seed, however it was no activity; and was reported on anti-bacterial activity of stink bean seed against P. acnes. Thus, further study on other extract, variety of plant and assay are need to conduct. [ABSTRACT FROM AUTHOR]