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New NIR spectroscopic probe with a large Stokes shift for Hg2+ and Ag+ detection and living cells imaging.
- Source :
-
Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy . Apr2022, Vol. 271, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
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Abstract
- A new NIR probe based on coumarin-dicyanooxanone dyad an extraordinarily large Stokes shift of 316 nm was developed for simultaneous fluorescence turn-on and colorimetric detection of Hg2+ and Ag+ in aqueous solution. Its application for in vitro U87MG cell imaging to detect Hg2+ was confirmed. [Display omitted] • A coumarin-dicyanooxanone dyad-based NIR fluorescent probe was synthesized. • • The probe displayed an extraordinarily large Stokes shift of 316 nm. • • It serves as colorimetric and fluorimetric probe for detection of Hg2+ and Ag+. • • The applicable pH for sensing Hg2+ by the probe is in a range of 2–12. • • Application of the probe for in vitro cell imaging to detect Hg2+ was confirmed. A new near-infrared (NIR) probe based on a coumarinyl ligand (CL) was designed and synthesized. The probe CL can be used for simultaneous fluorescent turn-on and colorimetric detection of Hg2+ and Ag+ in ethanol/water medium. Colorless solution of probe CL changed to light yellow or dark yellow after addition of Hg2+ or Ag+ ions. Meanwhile the maximum absorption band shifted from 379 nm to 404 nm and the intensity increased enormously (for Hg2+) or moderately (for Ag+). Probe CL displayed an extraordinarily large Stokes shift of 316 nm and addition of Hg2+ or Ag+ to probe CL induced enhancement in the intensity of fluorescence emission at 695 nm by 15 or 8 fold. The detection limit of CL for Hg2+ and Ag+ ions is 0.83 and 8.8 μM, respectively. The applicable pH for sensing Hg2+ by probe CL is in a broad range of 2–12. Application of probe CL for in vitro U87MG cell imaging to detect Hg2+ ions was confirmed. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 13861425
- Volume :
- 271
- Database :
- Academic Search Index
- Journal :
- Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy
- Publication Type :
- Academic Journal
- Accession number :
- 155341594
- Full Text :
- https://doi.org/10.1016/j.saa.2022.120916