Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells.

The article discusses a study on mapping megakaryocyte progenitors (MKPs) from pluripotent stem cells. It performs single-cell RNA sequencing in vitro differentiation and mapped it in vivo that identifies five surface markers, and culture optimization to increase MKP production. It concludes that in vitro in the MKP state mirrors the state in vivo thus preventing hemorrhage from platelet deficiency with platelet transfusions.