Skin mucus proteins of rainbow trout (Oncorhynchus mykiss) in response to mucosal vaccination and challenge with Flavobacterium psychrophilum.

Keywords: Flavobacterium psychrophilum; immersion challenge; immersion vaccination; proteomics; rainbow trout; skin mucus EN Flavobacterium psychrophilum immersion challenge immersion vaccination proteomics rainbow trout skin mucus 491 495 5 02/10/22 20220301 NES 220301 I Flavobacterium psychrophilum i is one of the most important pathogens affecting rainbow trout ( I Oncorhynchus mykiss i ) worldwide at the fry stage of their life cycle. Briefly, skin mucus was sampled by placing three fish (from each duplicate tank giving 2 pools of mucus/group) into a plastic bag containing 5 ml of Tris-buffered saline (TBS: 10 mM Tris base and 0.5 M NaCl, pH 7.5) and gently massaging for 2 min. In the current study, we examined the proteins of skin mucus of rainbow trout following immersion vaccination with I F. psychrophilum i . [Extracted from the article]