BODIPY-based near-infrared fluorescent probe for diagnosis drug-induced liver injury via imaging of HClO in cells and in vivo.

Drug-induced liver injury (DILI) as an acute liver disease has caused serious concern in public health problems. As a kind of reactive oxygen species, HClO has come into scientists' vision as a marker of DILI. Herein, we construct a BODIPY-based near-infrared (NIR) fluorescent probe (BDP-R-ClO) for the selective and sensitive response of hypochlorous acid. The probe could realize the rapid detection of HClO, with a 20-fold fluorescence enhancement at 661 nm and the detection value of which was only 1.8 nM. Moreover, BDP-R-ClO could diagnose and visualize DILI by monitoring the fluctuation of HClO concentration in vitro and in vivo , and evaluate the detoxification of hepatoprotective drugs. Through the visualization of HClO in the mice liver injury models, BDP-R-ClO not only proved its applicability but also indicated that it had great potential in the diagnosis and monitoring of HClO-related diseases. [Display omitted] ● A novel BODIPY-based NIR fluorescent probe for detection of HClO was developed. ● Probe exhibited favorable selectivity and sensibility towards HClO. ● Probe could be utilized to visualize HClO via fluorescence imaging in live cells and in vivo. ● Probe realized the diagnosis of APAP-induced hepatotoxicity, and evaluated the detoxification of hepatoprotective drugs. [ABSTRACT FROM AUTHOR]