不同果实形状番茄品种茎部内生细菌群落结构及代谢功能特征.

[Objective] To provide theoretical basis for developing a new tomato breeding evaluation system, the endophytic bacterial communities structure in stems of tomato varieties with different fruit shapes were analyzed, the correlation between endophytic bacteria and fruit shape was explored. [ Method] B ioinformatics analysis of the endophytic bacterial community structure and related metabolic functions in stems of different fruit shapes tomatoes ( ellipsoid, flat, long and round) were analyzed using lllumina Mi Seq sequencing technology. [ Result] A total of 31 phyla,84 orders,206 orders,347 families,655 genera,1045 species,and 1660 OTUs were identified from endophytic bacteria in stems of four fruit-shaped tomato varieties. However, there was no significant differences ( P > 0. 05) on the diversity and richness indices of the endophytic bacteria between each variety. At phylum level, Chloroflexi, Actinobacteriota, Bacteroidota, Firmicutes, Proteobacteria and Actinobaciota were the dominant endophytic bacteria in stems of four different fruit shapes tomato varieties. The composition of endophytic bacteria at phylum level was quite similar among four different fruit shapes tomato varieties. However, the proponions of different endophytic bacteria at phylum level in stems of four different frnit shapes tomato varieties were difference. At genus level, Ralstonia, Pseudonocardia, Brevibacterium, Brachybactenum, Bacillus and Rlwdococcus were the common dominant endophytic bacteria in stems of four different fruit shapes tomato varieties. PICRUSt functional prediction results showed that six types of biological metabolic pathways in the primary functional layer of endophytic bacterial genes could be found in stems of tomatoes with different fruit shapes, and the metabolic pathway was the main functions of endophytic bacteria in stems of four different fruit shapes of tomatoes. Meanwhile, although the types of predicted gene in secondary functional layer were similar, but their numbers were different in stems of different fruit shapes of tomatoes. [Conclusion] Although some common dominant endophytic bacteria at genus level could be found in stems of four different Jiuit shaped tomato varieties, but their proponions also showed in different ratios. Meanwhile, some unique dominant endophytic bacteria at genus level also could be detected in four different fruit shaped tomato varieties, respectively. It suggested that the unique dominant endophytic bacteria in stems of tomatoes at genus level and their functions, could be strongly related to the fruit shapes forming processes. It also indicates that the composition of endophytic bacteria in stems of tomatoes can be used as a bio-indicator with great potential for evaluating fruit shapes. [ABSTRACT FROM AUTHOR]