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Thread-based isotachophoresis coupled with desorption electrospray ionization mass spectrometry for clean-up, preconcentration, and determination of alkaloids in biological fluids.

Authors :
Chen, Liang
Ghiasvand, Alireza
Lam, Shing Chung
Rodriguez, Estrella Sanz
Innis, Peter C.
Paull, Brett
Source :
Analytica Chimica Acta. Feb2022, Vol. 1193, pN.PAG-N.PAG. 1p.
Publication Year :
2022

Abstract

A thread-based isotachophoresis method coupled with desorption electrospray ionization mass spectrometry (TB-ITP-DESI-MS) was developed and applied for clean-up, preconcentration, and determination of alkaloids in biological fluids. This simple approach enables the focusing and rapid analysis of analytes of interest in complex matrices that are otherwise challenging using direct ambient mass spectrometry. The TB-ITP platform components were rapidly and reproducibly fabricated at low-cost using 3D printing. A single string of nylon 6 thread was used as the electrophoresis substrate and a cotton knot, tied to the nylon thread, was used as the trapping zone of the ITP focused model analytes (coptisine, berberine and palmatine). The trapping efficiency was evaluated upon different commercially available threads with different chemical properties and cotton was selected as the best material due to its highest trapping efficiency and subsequent DESI-MS ionization efficiency. Up to 11.6-fold increase in signal to noise ratio (S/N) was obtained using the proposed method compared to direct DESI-MS detection, due to the reduced matrix interference and focusing. The results demonstrated that the TB-ITP-DESI-MS approach is a viable solution for the analysis of complicated biological fluid samples. [Display omitted] • Thread-based isotachophoresis applied to concentrate target small molecules. • Concentrated solute bands quantitatively trapped upon cotton knot applied to thread. • Knot used as substrate for ambient mass spectrometry detection. • Method applied to alkaloids in biological fluids (urine). [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00032670
Volume :
1193
Database :
Academic Search Index
Journal :
Analytica Chimica Acta
Publication Type :
Academic Journal
Accession number :
154718076
Full Text :
https://doi.org/10.1016/j.aca.2021.338810