ARTP-DES复合诱变选育高产纤维素酶突变菌株.

In order to select a high cellulase producing strain, Trichoderma pseudokoningii was used as the starting strain, the compound physical and chemical mutagenesis of normal temperature and pressure plasma (ARTP) and diethyl sulfate (DES) was used. The compound mutagenesis conditions were determined as ARTP irradiation time of 110 s and DES concentration of 0.8%. Congo red staining was used to screen the mutant strains, and the ratio of transparent circle to colony diameter of 19 mutant strains was greater than that of the original strain. The cellulase activity of 19 mutant strains was determined, and a strain AD-55 with higher cellulase activity was obtained. The filter paper enzyme activity, exonuclease activity and endonuclease activity were 2.16, 7.04 and 3.98 IU/mL, respectively. Through single factor and orthogonal experiments, the optimum enzyme production medium conditions were microcrystalline cellulose 1.25%, corn pulp powder 1.20% and (NH4)2SO4 0.20%. Under the condition, the filter paper enzyme activity, exonuclease activity and endonuclease activity of strain AD-55 were 2.89, 8.82 and 5.10 IU/mL, respectively. The strain AD-55 was genetically stable. [ABSTRACT FROM AUTHOR]