Nitric oxide inhibitory terpenes and its glycosides from Ainsliaea bonatii.

Seven new terpenes and its derivatives classified into a p -menthane glycoside (1), a guaianolide glycoside (2), three eudesmane and its glycosides (3 – 5), and two mono-terpene derivatives (9 and 10) were isolated from Ainsliaea bonatii , together with three known guaianolides (6 – 8). Their structures were elucidated by spectroscopic data analysis, and absolute configurations were determined by DP4+ probability analysis via calculated 13C NMR data of isomers. Compounds 6 and 9 showed nitric oxide (NO) inhibitory effects in LPS-induced RAW 264.7 macrophage cells with IC 50 values of 9.3 and 10.6 μ M, respectively. [Display omitted] • Seven new terpenes classified into mono-terpene, guaianolide and eudesmanolide types were isolated from Ainsliaea bonatii. • Their structures were elucidated based on spectroscopic analysis and quantum chemical calculations of 13C NMR data. • Compounds 6 and 9 showed NO inhibitory effects with IC 50 values of 9.3 and 10.6 μM, respectively. [ABSTRACT FROM AUTHOR]