Back to Search
Start Over
Homogeneous supported monolayer from microbial glycolipid biosurfactant.
- Source :
-
Journal of Molecular Liquids . Jan2022, Vol. 345, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- • A single liquid crystalline lamellar membrane of a glucolipid microbial biosurfactant can be supported onto a flat substrate. • Deposition is performed by dip-coating, an easy-to-implement physical method. • Dip-coating allows control of a single molecular layer (2.8 ± 1.0 nm) of the supported membrane. • The supported membrane is entirely glycosylated and crack-free on the support, from nm -to- cm scale. The development of supported glycosylated lipid layers is an important trend in the field of glyconanomaterials for their interest in understanding sugar-sugar and protein–sugar interactions, these being at the core of cellular, bacterial or viral adhesion. The conventional self-assembled monolayer (SAM) approach generally requires a thiolated glycoconjugate and a gold substrate. In this work, we show how glycolipid amphiphiles of natural origin, commonly known as microbial biosurfactants, can be easily deposited onto a substrate. Spontaneously produced by microorganisms but lacking a thiol group, one can take advantage of their self-assembly properties to prepare homogeneous supported lipid monolayers (SLM). We then choose a saturated glucolipid, G-C18:0, which forms a colloidal lamellar phase under diluted conditions. The lamellae can then be deposited onto a substrate (silicon, gold) using a physical method (dip coating). Dip coating is preferred over more classical deposition methods (Langmuir-Blodgett-LB-, vesicle fusion or spin-coating) because of its versatility, compatibility with aqueous solutions and robust control of the thickness below 10 nm. Defect-free glycosylated SLM from a microbial biosurfactant are then easily developed. A combination of ellipsometry, fluorescence microscopy, atomic force microscopy and infrared nanospectroscopy (AFMIR) show that the glycosylated SLM are defect-free, have a thickness of 2.8 ± 1.0 nm and they are highly homogeneous at scales going from the nm to cm. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01677322
- Volume :
- 345
- Database :
- Academic Search Index
- Journal :
- Journal of Molecular Liquids
- Publication Type :
- Academic Journal
- Accession number :
- 154503530
- Full Text :
- https://doi.org/10.1016/j.molliq.2021.117827