结直肠癌组织ANP32A、Ataxin-3、FHL1的表达及其与肝转移的关系研究.

Objective: To investigate the expression of acidic nuclear phosphoprotein 32A (ANP32A), Ataxin-3 and four half LIM domain proteins 1 (FHL1) in colorectal cancer and their relationship with liver metastasis. Methods: The protein levels of ANP32A, Ataxin-3 and FHL1 protein in 120 cases of colorectal cancer were detected by immunohistochemistry, the positive expression rate was analyzed. Among thems, 44 cases with liver metastasis were regarded as liver metastasis group, 76 cases without liver metastasis were re- garded as non liver metastasis group, the positive expression rates of ANP32A, Ataxin-3 and FHL1 were compared between the two groups, the influencing factors of colorectal cancer liver metastasis were analyzed, and the correlation between ANP32A, Ataxin-3 and FHL1 protein were analyzed. Results: The positive expression rate of ANP32A protein in colorectal cancer tissues was higher than that in adjacent tissues, and the positive expression rates of Ataxin-3 and FHL1 protein in colorectal cancer tissues were lower than those in adjacent tissues (P<0.05). Univariate analysis showed that the positive expression rate of ANP32A protein in liver metastasis group was significantly higher than that in non liver metastasis group, and the positive expression rates of Ataxin-3 and FHL1 protein in liver metastasis group were significantly lower than those in non liver metastasis group (P<0.05). The proportion of patients with low differentiation, T3-T4 invasion depth and lymph node metastasis in liver metastasis group was significantly higher than that in non liver metastasis group (P<0.05). Multivariate logistic regression analysis showed that positive expression of ANP32A protein, low differentiation of primary cancer, depth of invasion T3-T4 and lymph node metastasis were risk factors for liver metastasis of colorectal cancer (P<0.05). Positive expression of Ataxin-3 and FHL1 protein were protective factors for liver metastasis of colorectal cancer (P<0.05). Spearman correlation analysis showed that the positive expression rate of ANP32A was negatively correlated with the positive expression rate of Ataxin-3 and FHL1(P<0.05), and the positive expression rate of Ataxin-3 was positively correlated with the positive expression rate of FHL1 (P<0.05). Conclusion: The high expression of ANP32A and the low expression of Ataxin-3 and FHL1 are closely related to the occurrence and liver metastasis of colorectal cancer. Positive expression of ANP32A, low differentiation of primary cancer, depth of invasion T3-T4 and lymph node metastasis were risk factors for liver metastasis of colorectal cancer. Positive expression of Ataxin-3 and FHL1 protein were protective factors for liver metastasis of colorectal cancer. [ABSTRACT FROM AUTHOR]