HBV多聚酶基因P区单位点和多位点突变、低中高HBV DNA载量、轻中重度的CHB患者外周血T淋巴细胞亚群观察.

Objective To observe the alterations of peripheral blood T lymphocyte subsets in chronic hepatitis B patients with different mutations in P region of hepatitis B virus (HBV) polymerase gene, HBV DNA load, and disease conditions. Methods We selected 61 patients with chronic hepatitis B. Gene sequencing was used to confirm the mutations in the P region of the HBV polymerase gene, and another 30 healthy people were taken as controls. We compared the changes of CD3+, CD4+, and CD8+ T lymphocytes between patients with chronic hepatitis B before and after the mutations in the P region of the HBV polymerase gene and the controls. According to the number of resistant gene mutation sites in P region, they were divided into the single-point group (29 cases) and multi-point group (32 cases); according to HBV DNA loads, they were divided into the low-copy group (6 cases), medium-copy group (22 cases), and high-copy group (33 cases); according to the degree of disease, they were divided into the mild group (12 cases), moderate group (25 cases), and severe group (24 cases) . The changes in expression levels of CD3+, CD4+ and CD8+ in peripheral blood were compared. Results After HBV resistance gene mutation, the expression levels of CD3+, CD4+ and CD8+ T lymphocytes decreased in CHB patients (all P<0. 05), and the expression levels of CD3+ and CD4+ T lymphocytes in CHB patients were significantly lower than those before mutation (both P<0. 05) . There were no significant differences in the expression levels of CD3+, CD4+ or CD8+ T lymphocytes in CHB patients at different loci (all P>0. 05) . There were no significant differences in the expression levels of CD3+, CD4+ or CD8+ T lymphocytes in CHB patients with different HBV DNA load (all P> 0. 05) . Compared with moderate patients, the severe patients had lower CD3+ and CD8+ T lymphocytes (both P<0. 05) . Conclusions The consumption of CD3+ and CD4+ T lymphocytes in peripheral blood of CHB patients with drug resistance gene mutation increases. With the increase of resistant gene mutation sites, HBV DNA load increases, and the consumption of CD3+, CD4+, CD8+ T lymphocytes shows an increasing trend. With the aggravation of the disease, the consumption of CD3+ and CD4+ T lymphocytes increases, especially in the severe stage of the disease, and the consumption of CD8+ T lymphocytes also increases. [ABSTRACT FROM AUTHOR]