Back to Search
Start Over
Evaluation of recombinase-based isothermal amplification assays for point-of-need detection of SARS-CoV-2 in resource-limited settings.
- Source :
-
International Journal of Infectious Diseases . Jan2022, Vol. 114, p105-111. 7p. - Publication Year :
- 2022
-
Abstract
- • Point-of-need diagnostics are required to contain the spread of SARS-CoV-2. • Recombinase-based assays rapidly detect SARS-CoV-2 in clinical samples. • The two isothermal amplification methods tested showed promising diagnostic efficiency. • The incorporation of these techniques in a 'suitcase laboratory' may facilitate remote deployment. The democratization of diagnostics is one of the key challenges towards containing the transmission of coronavirus disease 2019 (COVID-19) around the globe. The operational complexities of existing PCR-based methods, including sample transfer to advanced central laboratories with expensive equipment, limit their use in resource-limited settings. However, with the advent of isothermal technologies, the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is possible at decentralized facilities. In this study, two recombinase-based isothermal techniques, reverse transcription recombinase polymerase amplification (RT-RPA) and reverse transcription recombinase-aided amplification (RT-RAA), were evaluated for the detection of SARS-CoV-2 in clinical samples. A total of 76 real-time reverse transcription PCR (real-time RT-PCR) confirmed COVID-19 cases and 100 negative controls were evaluated to determine the diagnostic performance of the isothermal methods. This investigation revealed equally promising diagnostic accuracy of the two methods, with a sensitivity of 76.32% (95% confidence interval 65.18–85.32%) when the target genes were RdRP and ORF1ab for RT-RPA and RT-RAA, respectively; the combination of N and RdRP in RT-RPA augmented the accuracy of the assay at a sensitivity of 85.53% (95% confidence interval 75.58–92.55%). Furthermore, high specificity was observed for each of the methods, ranging from 94.00% to 98.00% (95% confidence interval 87.40–9.76%). Considering the diagnostic accuracies, both RT-RPA and RT-RAA appear to be suitable assays for point-of-need deployment for the detection of the pathogen, understanding its epidemiology, case management, and curbing transmission. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COVID-19
*SARS-CoV-2
*COVID-19 pandemic
Subjects
Details
- Language :
- English
- ISSN :
- 12019712
- Volume :
- 114
- Database :
- Academic Search Index
- Journal :
- International Journal of Infectious Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 154436120
- Full Text :
- https://doi.org/10.1016/j.ijid.2021.11.007