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Two novel cyanobacterial α-dioxygenases for the biosynthesis of fatty aldehydes.
- Source :
-
Applied Microbiology & Biotechnology . Jan2022, Vol. 106 Issue 1, p197-210. 14p. - Publication Year :
- 2022
-
Abstract
- α-Dioxygenases (α-DOXs) are known as plant enzymes involved in the α-oxidation of fatty acids through which fatty aldehydes, with a high commercial value as flavor and fragrance compounds, are synthesized as products. Currently, little is known about α-DOXs from non-plant organisms. The phylogenic analysis reported here identified a substantial number of α-DOX enzymes across various taxa. Here, we report the functional characterization and Escherichia coli whole-cell application of two novel α-DOXs identified from cyanobacteria: CalDOX from Calothrix parietina and LepDOX from Leptolyngbya sp. The catalytic behavior of the recombinantly expressed CalDOX and LepDOX revealed that they are heme-dependent like plant α-DOXs but exhibit activities toward medium carbon fatty acids ranging from C10 to C14 unlike plant α-DOXs. The in-depth molecular investigation of cyanobacterial α-DOXs and their application in an E. coli whole system employed in this study is useful not only for the understanding of the molecular function of α-DOXs, but also for their industrial utilization in fatty aldehyde biosynthesis. Key points • Two novel α-dioxygenases from Cyanobacteria are reported • Both enzymes prefer medium-chain fatty acids • Both enzymes are useful for fatty aldehyde biosynthesis [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 106
- Issue :
- 1
- Database :
- Academic Search Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 154427834
- Full Text :
- https://doi.org/10.1007/s00253-021-11724-x