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Dendrimer‐Like Supramolecular Assembly of Proteins with a Tunable Size and Valency Through Stepwise Iterative Growth.
- Source :
-
Advanced Science . Dec2021, Vol. 8 Issue 24, p1-10. 10p. - Publication Year :
- 2021
-
Abstract
- The assembly of proteins in a programmable manner provides insight into the creation of novel functional nanomaterials for practical applications. Despite many advances, however, a rational protein assembly with an easy scalability in terms of size and valency remains a challenge. Here, a simple bottom‐up approach to the supramolecular protein assembly with a tunable size and valency in a programmable manner is presented. The dendrimer‐like protein assembly, simply called a "protein dendrimer," is constructed through a stepwise and alternate addition of a building block protein. Starting from zeroth‐generation protein dendrimer (pG0) of 27 kDa, the protein dendrimer is sequentially grown to pG1, pG2, pG3, to pG4 with a molecular mass of 94, 216, 483, and 959 kDa, respectively. The valency of the protein dendrimers at the periphery increases by a factor of two after each generation, allowing a tunable valency and easy functionalization. The protein dendrimers functionalizes with a targeting moiety and a cytotoxic protein cargo shows a typical feature of multi‐valency in the avidity and a highly enhanced cellular cytotoxicity, exemplifying their utility as a protein delivery platform. The present approach can be effectively used in the creation of protein architectures with new functions for biotechnological and medical applications. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MOLECULAR weights
*BUILDING additions
*PROTEINS
*DENDRIMERS
*MOIETIES (Chemistry)
Subjects
Details
- Language :
- English
- ISSN :
- 21983844
- Volume :
- 8
- Issue :
- 24
- Database :
- Academic Search Index
- Journal :
- Advanced Science
- Publication Type :
- Academic Journal
- Accession number :
- 154274286
- Full Text :
- https://doi.org/10.1002/advs.202102991