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Inositol 1,4,5-Trisphosphate Receptor Localization and Stability in Neonatal Card iomyocyte s Requires Interaction with Ankyrin-B.
- Source :
-
Journal of Biological Chemistry . 3/26/2004, Vol. 279 Issue 13, p12980-12987. 8p. 24 Color Photographs, 1 Diagram, 8 Graphs. - Publication Year :
- 2004
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Abstract
- The molecular mechanisms required for inositol 1,4,5trisphosphate receptor (InsP3R) targeting to specialized endoplasmic reticulum membrane domains are unknown. We report here a direct, high affinity interaction between InsP3R and ankyrin-B and demonstrate that this association is critical for InsP3R post-translational stability and localization in cultures of neonatal cardiomyocytes. Recombinant ankyrin-B membrane-binding domain directly interacts with purified cerebellar InsP3R (Kd = 2 nM). 220-kDa ankyrin-B co-immunoprecipitates with InsP3R in tissue extracts from brain, heart, and lung. Alanine-scanning mutagenesis of the ankyrin-B ANK (ankyrin repeat) repeat β-hairpin loop tips revealed that consecutive ANK repeat β-hairpin loop tips (repeats 22-24) are required for InsP3R interaction, thus providing the first detailed evidence of how ankyrin polypeptides associate with membrane proteins. Pulse-chase biosynthesis experiments demonstrate that reduction or loss of ankyrin-B in ankyrin-B (+/-) or ankyrin-B (-/-) neonatal cardiomyocytes leads to ∼3-fold reduction in half-life of newly synthesized InsP3R. Furthermore, interactions with ankyrin-B are required for InsP3R stability as abnormal InsP3R phenotypes, including mis-localization, and reduced halflife in ankyrin-B (+/-) cardiomyocytes can be rescued by green fluorescent protein (GFP)-220-kDa ankyrin-B but not by GFP-220-kDa ankyrin-B mutants, which do not associate with InsP3R. These new results provide the first physiological evidence of a molecular partner required for early post-translational stability of InsP3R. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00219258
- Volume :
- 279
- Issue :
- 13
- Database :
- Academic Search Index
- Journal :
- Journal of Biological Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 15413170
- Full Text :
- https://doi.org/10.1074/jbc.M313979200