Identification and characterization of SARS-CoV-2 clusters in the EU/EEA in the first pandemic wave: additional elements to trace the route of the virus.

A high-quality dataset of 3289 complete SARS-CoV-2 genomes collected in Europe and European Economic Area (EAA) in the early phase of the first wave of the pandemic was analyzed. Among all single nucleotide mutations, 41 had a frequency ≥ 1%, and the phylogenetic analysis showed at least 6 clusters with a specific mutational profile. These clusters were differentially distributed in the EU/EEA, showing a statistically significant association with the geographic origin. The analysis highlighted that the mutations C14408T and C14805T played an important role in clusters selection and further virus spread. Moreover, the molecular analysis suggests that the SARS-CoV-2 strain responsible for the first Italian confirmed COVID-19 case was already circulating outside the country. • Sequences alignment of a high-quality dataset of Sars-CoV-2 genomes showed some highly conserved genes. • Phylogenetic analysis revealed six clusters. The distribution of the clusters was associated with specific geographic areas. • The SARS-CoV-2 strain detected in the first COVID-19 case diagnosed in Northern Italy was already circulating outside the country. [ABSTRACT FROM AUTHOR]