围生期低剂量双酚A对子代雄鼠生精功能的影响.

Objective: To study the influence of perinatal exposure of bisphenol A (BPA) at a low dose on the spermatogenesis of male offspring rats. Methods: Pregnant rats were injected subcutaneously with BPA 2 μg/(kg·d) from gestation day 10 through lactation day 7, and the same volume of olive oil was used as the blank control. Their male offspring were served as the BPA group and the control group. Histological structure of the testis and the related regulation genes such as cyclin A1、cyclin B、c-jun and c-fos, as well as the FSH and LH, were tested, and the expressions POA-GnRH or ARC-kiss1 mRNAs were also examined in their male offspring during the postnatal day 18, 21, 24. Results: In comparison with the control group, the seminiferous tubular diameter, the number of round spermatids and the ratio of seminiferous tubule with round spermatids were significantly increased in PND24 BPA rats (P＜0.05). The expression levels of cyclin A1, c-jun and c-fos mRNAs in the testes were significantly elevated in PND21 BPA rats (P＜0.01). Similarly, the levels of plasma FSH and LH were increased in the PND21/24 BPA rats compared with the control rats (P＜0.05). But the levels of plasma T and E2 were no significant in the PND18/21/24 BPA rats and the control rats (P＞0.05). In comparison with control group, the expression levels of POA-GnRH mRNA and ARC-kiss1 mRNA were significantly increased in PND21 BPA rats (GnRH: P＜0.05; kiss1: P＜0.01). Conclusions: In addition to the direct effect of BPA on the testes of male offspring, the perinatal exposure to a low dose of BPA promotes spermatogenesis via advancing the pubertal activation of ARC -kisspeptin, and via further enhancing the expressions of meiotic regulation genes in the testes of male offspring. [ABSTRACT FROM AUTHOR]