基质金属蛋白酶3 基因多态性与膝骨性关节炎的遗传易感性.

BACKGROUND: Although great progress has been made in the etiology, development and treatment of knee osteoarthritis in recent years, its specific etiology and pathogenesis are still unclear. Among the pathogenic factors of knee osteoarthritis, genetic factors account for about 40%-60%, which are closely related to the incidence of knee osteoarthritis. OBJECTIVE: To investigate the relationship between matrix metalloproteinase-3 gene polymorphism and onset of knee osteoarthritis in Han population in north China. METHODS: We recruited 100 male patients with knee osteoarthritis and 197 healthy men admitted at the Affiliated Hospital of Inner Mongolia Medical University between October 2018 and October 2020. All the subjects were from the Han group. We used commercial kits to extract the blood genomic DNAs. Eight single nucleotide polymorphisms of matrix metalloproteinase-3 gene, including rs639752, rs650108, rs520540, rs646910, rs602128, rs679620, rs678815, and rs522616, were genotyped by using the Sequenom MassARRAY RS1000 platform. Unconditional Logistic regression analysis was used to calculate the correlation between single nucleotide polymorphisms and knee osteoarthritis under five genetic models (codominant, dominant, recessive, overdominant and additive models). RESULTS AND CONCLUSION: (1)In the Pearson chi-square test, all loci had no correlation with the risk of knee osteoarthritis under the allelic model. (2) Through the association analysis, we identified that four single nucleotide polymorphisms (rs639752, rs520540, rs602128, and rs679620) in the matrix metalloproteinase-3 gene in the dominant and over-dominant models were associated with the increased risk of knee osteoarthritis. (3)Our study preliminarily revealed that four single nucleotide polymorphisms (rs639752, rs520540, rs602128, and rs679620) in the matrix metalloproteinase-3 gene can increase the incidence of knee osteoarthritis in men of Han ethnics in north China. However, the results we identified here need to be confirmed in further large-scale studies. [ABSTRACT FROM AUTHOR]