Verification of Real-Time Microbial Monitoring Technology Based on the Principle of Laser-Induced Fluorescence Under USP <1223> Standard and Prospective Application in Our Hospital PIVAS Clean Room.

The comparison of laser induced fluorescence real-time monitoring technology application versus traditional planktonic bacteria and dust particle testing methods in the monitoring of indoor air microorganism in our hospital dentralized drug dispensing center was carried out, so as to provide technical support for the real-time monitoring and early warning of PIVAS to the planktonic bacteria and dust particles. First of all, the aerosol generator was used to conduct bacteria generation experiments, and the feasibility of the substituted method was verified using 405 nm laser induced fluorescence technology and the traditional air plankton sampler. Secondly, the actual application effects of the real-time monitoring system of biological aerosol, plankton-sampler and dust particle counter of laser-induced fluorescence technology were compared and evaluated in the PIVAS, 300,000-level buffer area and non-clean control area in our hospital. The real-time monitoring system of bioaerosol using laser-induced fluorescence technology has a significant sensitivity advantage over the traditional plankton-sampling apparatus, and its verification indexes, all meet the relevant requirements under USP <1223 >. There is no significant difference in the dust particle counting efficiency of the real-time monitoring system of bio-aerosol with laser induced fluorescence technology in PIVAS clean area. Therefore, the real-time monitoring system of bio-aerosol with laser induced fluorescence technology can be applied to the real-time monitoring of environmental microbial pollution in PIVAS clean room, and it has a broad application prospect in PIVAS environmental control and even hospital environmental control. In the future, it can be effectively combined with the PIVAS Intelligent Operation Service Platform in hospital. [ABSTRACT FROM AUTHOR]