Back to Search
Start Over
New microfluidic paper-based analytical device for iron determination in urine samples.
- Source :
-
Analytical & Bioanalytical Chemistry . Dec2021, Vol. 413 Issue 30, p7463-7472. 10p. - Publication Year :
- 2021
-
Abstract
- Iron is an important micronutrient involved in several mechanisms in the human body and can be an important biomarker. In this work, a simple and disposable microfluidic paper-based analytical device (µPAD) was developed for the quantification of iron in urine samples. The detection was based on the colorimetric reaction between iron(II) and bathophenanthroline and the reduction of iron(III) to iron(II) with hydroxylamine. The developed µPAD enabled iron determination in the range 0.07–1.2 mg/L, with a limit of detection of 20 µg/L and a limit of quantification of 65 µg/L, thus suitable for the expected values in human urine. Additionally, targeting urine samples, the potential interference of the samples color was overcome by incorporating a sample blank assessment for absorbance subtraction. Stability studies revealed that the device was stable for 15 days prior to usage and that the formed colored product was stable for scanning up to 3 h. The accuracy of the developed device was established by analyzing urine samples (#26) with the developed µPAD and with the atomic absorption spectrometry method; the relative deviation between the two sets of results was below 9.5%. [ABSTRACT FROM AUTHOR]
- Subjects :
- *COLORIMETRY
*IRON
*URINALYSIS
Subjects
Details
- Language :
- English
- ISSN :
- 16182642
- Volume :
- 413
- Issue :
- 30
- Database :
- Academic Search Index
- Journal :
- Analytical & Bioanalytical Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 153819757
- Full Text :
- https://doi.org/10.1007/s00216-021-03706-9