饲粮中添加染料木黄酮对产蛋后期种母鸡繁殖性能的影响.

[Objectives] The experiment was conducted to study the regulatory effect of genistein on the reproductive performance of breeder hens during the late laying period, in an effort to lay the foundation for the development of feed additives for breeder hens. [Methods] 180 58-week-old Qiling breeder hens with similar body weight and physiological status were randomly divided into three groups: control group, low dose genistein(L-Gen) group and high dose genistein(H-Gen) group, with 5 replicate per group. The preparation period lasted for 2 weeks, while the formal experimental period lasted for 4 weeks. Hens in all groups were fed with the corn soybean meal basal diet for 2-week preparation period. During the formal experimental period, the control group was fed with the basal diet. The L-Gen and H-Gen groups were fed with 20 and 40 mg·kgkg-1 genistein, respectively. [Results] The results showed that adding 20 mg·kgkg-1 genistein in the diet significantly increased the average egg weight, eggshell thickness and GnRH mRNA expression in the hypothalamus(P<0.05); 40 mg·kgkg-1 genistein treatment significantly increased the egg production rate and average egg weight in the 3rd and 4th weeks of the experiment, and the increased level of estrogen and progesterone in the serum(P<0.05). In addition, the egg yolk color grade and eggshell strength of L-Gen and H-Gen groups were higher than those of the control group(P<0.05). Furthermore, genistein treatment significantly increased the SOD activity and T-AOC content(P<0.05), and up-regulated the mRNA expression of ERα and FSHR in the yolk follicle(P<0.05). [Conclusions] In conclusion, genistein can activate the hypothalamus-pituitary-gonad axis, enhance the antioxidant capability and reproductive hormone secretion of the ovary, and improve the reproductive performance of breeder hens in the late laying period. [ABSTRACT FROM AUTHOR]