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α-Glucosidase inhibitors from brown rice bound phenolics extracts (BRBPE): Identification and mechanism.
- Source :
-
Food Chemistry . Mar2022, Vol. 372, pN.PAG-N.PAG. 1p. - Publication Year :
- 2022
-
Abstract
- [Display omitted] • Affinity-ultrafiltration strategy was used to figure α-glucosidase inhibitors. • 7 potential α-glucosidase inhibitors were identified from brown rice. • The type of inhibition of the inhibitors against α-glucosidase were measured. • The inhibitory mechanisms of 7 inhibitors were studied by molecular simulation. Brown rice bound phenolics extracts (BRBPE) have been reported to possess α-glucosidase inhibitory effects, the specific enzyme inhibitors involved in this process were unknown. Here, α-glucosidase inhibitors in BRBPE were screened using bioaffinity ultrafiltration methods, and seven phenolic compounds – three monomers (p -coumaric acid, ferulic acid and methyl ferulate), three dimers (8-5′, 5-5′ and 8- O -4′ diferulic acid) and a trimer (5-5′/8- O -4″ dehydrotriferulic acid) were identified as exact inhibitors, among which 5-5′/8- O -4″ dehydrotriferulic acid and 5-5′diferulic acid exhibited the best inhibitory activity. Enzyme kinetic analysis suggested that the inhibitory mechanism of these seven inhibitors including competitive, noncompetitive, uncompetitive and mixed manner. Molecular docking analysis revealed that the seven inhibitors bind with α-glucosidase mainly by hydrogen bonding interaction, hydrophobic force and ionic bond. Molecular dynamics simulation further explored the structure and molecular property of phenolic-glucosidase complex. This work provided a deep insight into brown rice bound phenolics acting as potent α-glucosidase inhibitors. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03088146
- Volume :
- 372
- Database :
- Academic Search Index
- Journal :
- Food Chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 153604892
- Full Text :
- https://doi.org/10.1016/j.foodchem.2021.131306