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Dynamic Hormone Gradients Regulate Wound-Induced de novo Organ Formation in Tomato Hypocotyl Explants.
- Source :
-
International Journal of Molecular Sciences . Nov2021, Vol. 22 Issue 21, p11843. 1p. - Publication Year :
- 2021
-
Abstract
- Plants have a remarkable regenerative capacity, which allows them to survive tissue damage after biotic and abiotic stresses. In this study, we use Solanum lycopersicum 'Micro-Tom' explants as a model to investigate wound-induced de novo organ formation, as these explants can regenerate the missing structures without the exogenous application of plant hormones. Here, we performed simultaneous targeted profiling of 22 phytohormone-related metabolites during de novo organ formation and found that endogenous hormone levels dynamically changed after root and shoot excision, according to region-specific patterns. Our results indicate that a defined temporal window of high auxin-to-cytokinin accumulation in the basal region of the explants was required for adventitious root formation and that was dependent on a concerted regulation of polar auxin transport through the hypocotyl, of local induction of auxin biosynthesis, and of local inhibition of auxin degradation. In the apical region, though, a minimum of auxin-to-cytokinin ratio is established shortly after wounding both by decreasing active auxin levels and by draining auxin via its basipetal transport and internalization. Cross-validation with transcriptomic data highlighted the main hormonal gradients involved in wound-induced de novo organ formation in tomato hypocotyl explants. [ABSTRACT FROM AUTHOR]
- Subjects :
- *ROOT formation
*TOMATOES
*AUXIN
*TRANSCRIPTOMES
*HORMONES
*PLANT hormones
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Volume :
- 22
- Issue :
- 21
- Database :
- Academic Search Index
- Journal :
- International Journal of Molecular Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 153600668
- Full Text :
- https://doi.org/10.3390/ijms222111843