人参皂苷 Rg1 对幼龄大鼠缺氧缺血性脑损伤和 神经元凋亡的保护作用.

Objective To explore the effects of ginsenoside Rg1 （ginsenoside Rg1） on cell apoptosis， oxidative stress and inflammation in young rats with hypoxic-ischemic brain damage （HIBD）.Methods Young Wistar rats were divided into healthy control group， model group （HIBD）， and HIBD+ginsenoside Rg1 group. Jumping test was used to detect the number of mistakes in each group of rats， brain tissue wet and dry weight method was used to calculate the brain water content and brain index of each group of rats. Hematoxylin-eosin （HE） staining was used to observe the degree of brain damage； TUNEL staining to observe the apoptosis level of brain tissue； Nissl body staining to detect neuronal cell apoptosis； Western blot to detect brain tissue Bax/Bcl-2， caspase-3， caspase-9 protein expressions； malondialdehyde （MDA） and superoxide dismutase （SOD） activity detection kit to detect MDA and SOD content， respectively； and ELISA to detect peripheral blood IL-6， iNOS， IL-4 contents. Results Compared with the model group， 20 mg/kg and 40 mg/kg ginsenoside Rg1 significantly reduced the number of mistakes in the jumping-off test of HIBD young rats， and brain index brain water rate and neuronal cell apoptosis （P<0.05）； alleviated brain tissue damage； down-regulated the protein expression levels of Bax/Bcl-2， cleaved cas9/cas9 and cleaved cas3/cas3 （P<0.05）； reduced IL-6 and iNOS protein content； and increased IL-4 protein content （P<0.05）.Conclusion Ginsenoside Rg1 improves brain tissue damage in young HIBD mice and inhibits neuronal cell apoptosis. [ABSTRACT FROM AUTHOR]