Extraction and enrichment of furanoid glycosylated diterpenes present in green coffee beans: New methodology in the QuEChERS concept.

[Display omitted] • Furanoid glycosylated diterpenes (FGD) from coffee was analyzed by TLC-MS. • A methodology for extracting and detecting FGD was developed. • Four FGD were detected after concentration step in macroporous polymeric resin. • QuEChERS allowed less consumption of solvents and reagents at FGD analysis in coffee. Four furanoid glycosylated diterpenes (mascaroside I, mascaroside III, mascaroside IV and mascaroside V) were extracted from green grains of Coffea arabica after clean up steps with organic solvents (chloroform/acetone and 80 % ethanol) and concentrated with the aid of macroporous polymeric resins (XAD-2) in column. The identification of compounds has been extensively tested with the aid of hyphenated spectroscopic techniques, such as TLC-MS and ESI-HRMS. The methodology employed was shown to be efficient (81 % recovery after column) and robust (molecular ions of compounds with error <5 ppm), fitting the QuEChERS concept. [ABSTRACT FROM AUTHOR]