Complete Thermal-Unfolding Profiles of Oxidized and Reduced Cytochromes c.

This article focuses on thermal unfolding profiles. An electron-transfer protein, soluble monoheme cytochrome c (cyt c) is among the essential redox proteins sustaining biological activity. Another fundamental way to elucidate the redox function of cyt c is to characterize its thermo-dynamic property of both oxidized and reduced forms because the redox potential is derived from the difference between these two redox states. Conventionally, there are two protein thermal-unfolding experiments. First, differential scanning calorimetric (DSC) measurements can be carried out at more than 100 degree celcius.