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Metabolic channeling: predictions, deductions, and evidence.
- Source :
-
Molecular Cell . Sep2021, Vol. 81 Issue 18, p3775-3785. 11p. - Publication Year :
- 2021
-
Abstract
- With the elucidation of myriad anabolic and catabolic enzyme-catalyzed cellular pathways crisscrossing each other, an obvious question arose: how could these networks operate with maximal catalytic efficiency and minimal interference? A logical answer was the postulate of metabolic channeling, which in its simplest embodiment assumes that the product generated by one enzyme passes directly to a second without diffusion into the surrounding medium. This tight coupling of activities might increase a pathway's metabolic flux and/or serve to sequester unstable/toxic/reactive intermediates as well as prevent their access to other networks. Here, we present evidence for this concept, commencing with enzymes that feature a physical molecular tunnel, to multi-enzyme complexes that retain pathway substrates through electrostatics or enclosures, and finally to metabolons that feature collections of enzymes assembled into clusters with variable stoichiometric composition. Lastly, we discuss the advantages of reversibly assembled metabolons in the context of the purinosome, the purine biosynthesis metabolon. The channeling of metabolic intermediates can increase pathway fluxes and protect metabolites. Mechanisms of channeling include direct physical tunnels, electrostatic trapping and enclosures, and membrane-less metabolic compartments or "metabolons." We highlight examples of the above classes, finally focusing on the purinosome to discuss the advantages of dynamically assembled metabolons. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10972765
- Volume :
- 81
- Issue :
- 18
- Database :
- Academic Search Index
- Journal :
- Molecular Cell
- Publication Type :
- Academic Journal
- Accession number :
- 152554897
- Full Text :
- https://doi.org/10.1016/j.molcel.2021.08.030