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Detection of exosomes via an electrochemical biosensor based on C60-Au-Tb composite.
- Source :
-
Microchemical Journal . Nov2021, Vol. 170, pN.PAG-N.PAG. 1p. - Publication Year :
- 2021
-
Abstract
- Schematic illustration of electrochemical detection biosensor of exosomes based on C 60 -Au-Tb as signal marker. [Display omitted] • A novel approach based on C 60 -Au-Tb for exosome detection is developed for the first time. • The limit of detection of this sensor is as low as 2.67 × 104 exosomes/mL. • The proposed sensor has the advantages of high sensitivity, high selectivity, simplicity and low cost. • This strategy may hold great potential in biomedical research and clinical diagnosis. As a new serum biomarker, the sensitive detection of exosomes is significant for the early diagnosis of diseases. In this paper, a novel electrochemical biosensor for the sensitive detection of exosomes was constructed utilizing a novel C 60 hybrid as a tracer. Firstly, the epidermal growth factor receptor (EGFR) antibody was immobilized on the electrode surface via Au-NH 2 bonding with Nafion AuNPs substrate. After specific capture of exosomes, C 60 -Au-Tb was grafted onto the surface of exosomes through "carboxyl-Zr4+-phosphate" chemistry, causing a change in the current signal. Under the optimal conditions, there was a linear relationship between the SWV signal and the logarithm of exosomes concentration from 5 × 104 exosomes/mL to 5 × 109 exosomes/mL, and the detection limit was 2.67 × 104 exosomes/mL. In addition, the biosensor had the advantages of high specificity, strong anti-interference ability, good repeatability, and stability. In conclusion, this method had great potential in diagnosing early cancers associated with exosomes. [ABSTRACT FROM AUTHOR]
- Subjects :
- *EXOSOMES
*BIOSENSORS
*EPIDERMAL growth factor receptors
Subjects
Details
- Language :
- English
- ISSN :
- 0026265X
- Volume :
- 170
- Database :
- Academic Search Index
- Journal :
- Microchemical Journal
- Publication Type :
- Academic Journal
- Accession number :
- 152368966
- Full Text :
- https://doi.org/10.1016/j.microc.2021.106772