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Mapping the spatial distribution of T cells in repertoire dimension.
- Source :
-
Molecular Immunology . Oct2021, Vol. 138, p161-171. 11p. - Publication Year :
- 2021
-
Abstract
- • Anatomical and physiological niches had significant influence on the distribution of an organism's TCR repertoire. • T cell receptor repertoire diversity and clonal expansion varied by anatomical location. • Small intestinal CD4 + T cell repertoire harbored highly expanded public clonotypes. T cells mediate adaptive immunity in diverse anatomic compartments through recognition of specific antigens via unique T cell receptor (TCR) structures. However, little is known about the spatial distribution of an organism's TCR repertoire. Here, using high-throughput TCR sequencing (TCRseq), we investigated the TCR repertoires of sixteen tissues in healthy C57B/L6 mice. We found that TCR repertoires generally classified into three categories (lymph nodes, non-lymph node tissues and small intestine) based on sequence similarity. Clonal distribution and diversity analyses showed that small intestine compartment had a more skewed repertoire as compared to lymph nodes and non-lymph node tissues. However, analysis of TRBV and TRBJ gene usage across tissue compartments, as well as comparison of CDR3 length distributions, showed no significant tissue-dependent differences. Interestingly, analysis of clonotype sharing between mice showed that although non-redundant public clonotypes were found more easily in lymph nodes, small intestinal CD4 + T cells harbored more abundant public clonotypes. These findings under healthy physiological conditions offer an important reference dataset, which may contribute to our ability to better manipulate T cell responses against infection and vaccination. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01615890
- Volume :
- 138
- Database :
- Academic Search Index
- Journal :
- Molecular Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 152204510
- Full Text :
- https://doi.org/10.1016/j.molimm.2021.08.009