Comparative analysis of paddy straw-degrading consortia in China using high-throughput sequencing.

Nine paddy straw-degrading microbial consortia were isolated from 253 paddy straw samples from northern China and the Yangtze River Valley, which are two major rice production regions in China. The microbial consortia completely degraded filter paper within 15 d at 25 °C. The V4 region of the microbial consortia 16S rRNA gene was amplified, and the amplicons were sequencing using the Illumina Miseq high-throughput platform. Compilobacterota and Bacteroidota were the predominant phyla in the samples. At the genus level, the relative abundance of Macellibacteroides was the highest in Y2, and Arcobacter was predominant in the other samples. Analysis of the microbial community structure revealed that the diversity and richness in samples from the Yangtze River Valley were significantly higher compared with those of the samples from northern China, and the community structure of the four microbial consortia from the northern region had higher similarity than the five consortia from the Yangtze River Valley. • Campilobacterota and Bacteroidetes were the predominant phyla of microbial consortia. • The major genera in the two regions were significantly different. • Pseudomonas , Comamonas , Fibrobacter , Sphingobacterium , Ruminiclostridium , and Ruminococcus are the functional bacteria. • The northern bacterial community richness and diversity were lower than the Yangtze River Valley. • The northern microbial community structure was higher in similarity than the Yangtze River Valley. [ABSTRACT FROM AUTHOR]