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Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels.

Authors :
Stevens, Sharon R.
Longley, Colleen M.
Yuki Ogawa
Teliska, Lindsay H.
Arumanayagam, Anithachristy S.
Nair, Supna
Oses-Prieto, Juan A.
Burlingame, Alma L.
Cykowski, Matthew D.
Mingshan Xue
Rasband, Matthew N.
Source :
eLife. 7/5/2021, p1-32. 32p.
Publication Year :
2021

Abstract

Neuronal ankyrins cluster and link membrane proteins to the actin and spectrin-based cytoskeleton. Among the three vertebrate ankyrins, little is known about neuronal Ankyrin-R (AnkR). We report AnkR is highly enriched in Pv+ fast-spiking interneurons in mouse and human. We identify AnkR-associated protein complexes including cytoskeletal proteins, cell adhesion molecules (CAMs), and perineuronal nets (PNNs). We show that loss of AnkR from forebrain interneurons reduces and disrupts PNNs, decreases anxiety-like behaviors, and changes the intrinsic excitability and firing properties of Pv+ fast-spiking interneurons. These changes are accompanied by a dramatic reduction in Kv3.1b K+ channels. We identify a novel AnkR-binding motif in Kv3.1b, and show that AnkR is both necessary and sufficient for Kv3.1b membrane localization in interneurons and at nodes of Ranvier. Thus, AnkR regulates Pv+ fast-spiking interneuron function by organizing ion channels, CAMs, and PNNs, and linking these to the underlying β1 spectrin-based cytoskeleton. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
2050084X
Database :
Academic Search Index
Journal :
eLife
Publication Type :
Academic Journal
Accession number :
152065334
Full Text :
https://doi.org/10.7554/eLife.66491