川芎白芷对过敏性皮炎模型的血流变及炎性因子表达影响的实验研究.

Objective: To study the intervention effect of Angelica Ligusticum chuanxiong on the tissues of allergic dermatitis from the perspective of subcutaneous blood rheology and inflammatory factor expression. Methods: 60 male pure white guinea pigs were randomly divided into control group, model group, Angelica dahurica group, Ligusticum chuanxiong group, Angelica combined Ligusticum chuanxiong group and positive drug group. Except for the normal group, the allergic contact subcutaneous inflammation model was established by 2.4-dinitrochlorobenzene method in all experimental groups except the normal group. After successful modeling, each group was designed to smear each group of drugs at inflammation sites. Four hours after the last administration, the blood flow changes in the affected ear of guinea pigs in each group were detected by laser Doppler flow imaging system. The ear tissues of guinea pigs in each experimental group were taken and pathological sections stained with HE were prepared. The inflammatory indicators IL-1beta, TNF-alpha, LTB4, LTD4, PGE in ear tissues were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the normal group, the blood flow of the ear branch trunk and terminal vessels of the sensitized model group increased sharply, and the blood flow of the ear vessels of each administration group was significantly lower than that of the model group. in which the combination of Ligusticum Chuanxiong and Angelica dahuricae had the most significant effect on the blood flow of inflamed tissue branches and trunks; the level of related inflammatory factors in the model is significantly increased, the inflammatory factor indexes of each administration group are significantly decreased, and the levels of LTB4, LTD4, PGD2, and PGE2 are decreased. Compared with the Angelicae group, the Ligusticum chuanxiong group had more significant effects on inhibiting the leukotriene indicators LTB4 and LTD4, and the Angelicae had more obvious effects on IL-1茁and IL-8. The combined use of Ligusticum chuanxiong and Angelicae group had better effects than the chuanxiong and Angelicae single-use group. Conclusion: Ligusticum chuanxiong Angelica has a therapeutic effect on the acute subcutaneous inflammation model. It can inhibit the inflammation in the tissue, reduce the release of prostaglandins and leukotrienes, and improve the hemorheological response caused by inflammation. The mechanism of action of Chuanxiong Angelica on acute subcutaneous inflammation is different, and it has a synergistic effect in inhibiting inflammatory mediators of leukotrienes and interleukins. [ABSTRACT FROM AUTHOR]