Bioanalytical Analysis of Small-Molecule Drugs and Metabolites in Physiological Samples by LC-MS, Part 2: Sample Preparation.

This article is the second of a series of four white papers on the bioanalysis of small-molecule drugs and metabolites by liquid chromatography-mass spectrometry (LC-MS). Part 1 was an overview of the fundamentals and regulations. This installment, part 2, is on sample preparation. Part 3 will cover method development, optimization, and best practices, and part 4 discusses method validation for regulated bioanalysis. This section provides an overview of sample preparation fundamentals, best practices, and modern trends. A sample preparation method development case study illustrates these practices and concepts. [ABSTRACT FROM AUTHOR]