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pH-Dependent Flavin Adenine Dinucleotide and Nicotinamide Adenine Dinucleotide Ultraviolet Resonance Raman (UVRR) Spectra at Intracellular Concentration.

Authors :
Merk, Virginia
Speiser, Eugen
Werncke, Wolfgang
Esser, Norbert
Kneipp, Janina
Source :
Applied Spectroscopy. Aug2021, Vol. 75 Issue 8, p994-1002. 9p.
Publication Year :
2021

Abstract

The ultraviolet resonance Raman spectra of the adenine-containing enzymatic redox cofactors nicotinamide adenine dinucleotide and flavin adenine dinucleotide in aqueous solution of physiological concentration are compared with the aim of distinguishing between them and their building block adenine in potential co-occurrence in biological materials. At an excitation wavelength of 266 nm, the spectra are dominated by the strong resonant contribution from adenine; nevertheless, bands assigned to vibrational modes of the nicotinamide and the flavin unit are found to appear at similar signal strength. Comparison of spectra measured at pH 7 with data obtained pH 10 and pH 3 shows characteristic changes when pH is increased or lowered, mainly due to deprotonation of the flavin and nicotinamide moieties, and protonation of the adenine, respectively. Graphical Abstract [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00037028
Volume :
75
Issue :
8
Database :
Academic Search Index
Journal :
Applied Spectroscopy
Publication Type :
Academic Journal
Accession number :
151649584
Full Text :
https://doi.org/10.1177/00037028211025575