Comparison of five membrane filters to collect bioaerosols for airborne microbiome analysis.

Aims: Recovering DNA of airborne micro‐organisms (AM) from air is a challenging task. We compared five membrane filters for bioaerosol sampling—mixed cellulose ester (MCE), polyethersulfone (PES), polyamide (PA), polytetrafluorethylene (PTFE) and polyvinylidene fluoride (PVDF)—based on their bacterial, fungal and eukaryotic DNA recoveries. Methods and results: Bacterial, fungal and eukaryotic populations were quantified using quantitative PCR. With a bacterial consortium, PTFE exhibited the best recovery efficiency (113%), followed by PA (92%), PES (86%), MCE (48%) and PVDF (1%). When filters were compared with air, PA was used as a control to normalize results from the others. The bacterial, fungal and eukaryotic DNA recovery ratios were markedly greater in PES (9·3, 11·5 and 10·3 respectively) than in the remaining. Eukaryotic MiSeq sequencing revealed that PES recovered a more diverse and considerably richer assemblage (richness ratios, 4·97 vs ≤ 1·16 for PES vs the others). Rank abundance distribution analysis showed that distribution tails were longer (>4 times) in PES, but these did not differ between the remaining and PA. Community comparison showed that PES exhibited a lower variation across trials than the PA, while the remaining did not. Conclusions: PES filter markedly outperformed the other filters in quantitative and qualitative recovery of AM. Significance and Impact of the Study: Our findings demonstrated the importance of filter selection for sampling AM. [ABSTRACT FROM AUTHOR]