CRIOPRESERVAÇÃO DE CÉLULASTRONCO DE ORIGEM DENTÁRIA: UMA REVISÃO DE LITERATURA.

Introduction: Dental tissues are an accessible source of mesenchymal stem cells that can be useful for the treatment of various clinical diseases. Thus, the interest and the need to ensure an efficient way to preserve stem cells of dental origin for future applications led to the development of cryopreservation methods, a technique by which low temperatures are applied to reverse, in a reversible and controlled manner, biological functions of living cells and tissues. Objective: To perform a literature review about the cryopreservation of stem cells of dental origin, emphasizing characteristics, principles, existing protocols, and effects of this method on the biological properties of these cells. Methodology: This study consisted of a literature review based on information from 54 scientific articles published between 2005 and 2019 and consulted in online databases (PubMed, SciELO, and Google Scholar), using the following descriptors: Cryopreservation, Dental stem cells, and Dental cryopreservation. Results: Stem cells of dental origin seem to maintain their biological characteristics, such as viability rate, cell proliferation, and ample capacity for differentiation even after cryopreservation. Also, magnetic cryopreservation presents itself as a promising protocol for the conservation of dental stem cells. Conclusion: Cryopreservation is an effective technique for the long-term storage of stem cells derived from dental tissues. However, additional studies should be carried out to develop more standardized and safer cryopreservation protocols that do not affect the biological properties of dental stem cells. [ABSTRACT FROM AUTHOR]