非生物胁迫对转基因抗虫棉Bt蛋白表达量的影响.

[Objective] To study the effect of abiotic stress on the expression of Bt protein in transgenic insect-resistant cotton. [Method] The seedlings of two transgenic insect-resistant cotton varieties Zhongmian 45 (ZGK9822) and Ekang insect cotton 1 (GK19) were treated with low temperature (4℃), high temperature (37℃), 0.1, 0.2, 0.5 mol· L -1 NaCl, mild drought, moderate drought, and severe drought 8 abiotic stress treatments. [Results] Low temperature and high temperature stress caused the Bt protein content in the roots, stems and leaves of the two insect-resistant cotton varieties to decrease to varying degrees. Among them, the Bt protein content of each tissue under low temperature stress was higher than that of high temperature stress; Compared with CK, the content of Bt protein in stems and leaves showed a trend of first decreasing and then increasing. In roots, stems and leaves of different degrees of drought stress, the change trend of Bt protein content was consistent with salt stress. [Conclusion] Abiotic stress seriously affects the expression of Bt protein in transgenic insect-resistant cotton, resulting in dynamic changes in the expression of Bt protein. When planting transgenic insect-resistant cotton in the field, attention should be paid to the impact of environmental factors. [ABSTRACT FROM AUTHOR]