In Vitro Activity of Eravacycline in Combination with Colistin Against OXA-type Carbapenemase Producing Klebsiella pneumoniae Isolates.

Objective: The synergistic activity of eravacycline in combination with colistin on Oxacillinase (OXA) producing carbapenem-resistant Klebsiella pneumoniae isolates (CR-Kp) was evaluated in this study. Methods: Minimum inhibitory concentrations of meropenem were determined by the broth microdilution method. All strains screened for carbapenemase activity by PCR. Chequerboard assay and time-kill analysis were used to assess potential synergy. Results: Synergistic activity was found in 40 % of the strains by chequerboard assay. No antagonism was detected. Comparing to colistin alone at subinhibitory concentrations synergistic and bactericidal activity was observed when it combined with eravacycline. A Similar activity was also observed in colistinresistant CR-Kp isolates. Conclusion: Our results indicate that Eravacycline and colistin combination may be a potential therapeutic option for the treatment of CR-Kp infections. [ABSTRACT FROM AUTHOR]