对Ghrelin/GHSR-la/GABA通路关键因子在胃食管反流病 肝郁证模型中表达水平的研究.

Objective To investigate the expression levels of Ghrelin/GHSR-la/GABA pathway key factors in rat model of gastroesophageal reflux disease (GERD) with liver constraint pattern. Methods 55 SD rats were randomly divided into the normal group, sham operation group, GERD group, liver constraint pattern group, and GERD with liver constraint pattern group, with 11 rats in each group. The GERD models were established by esophagogastroduodenal anastomosis (EGDA), the liver constraint pattern models were established by chronic unpredictable stimulation (CUS), and the GERD with liver constraint pattern models were established by EGDA combined with CUS. Sucrose preference test, open field test, and HE pathological observation of esophageal epithelium were performed. Ghrelin levels in serum and gastric tissue, GABA and GHSR-la expressions in the hypothalamus was detected. Results Compared with the normal group and the GERD group respectively, the sucrose preference percentage and the total number of grids crossed (P <0. 01) and the time spent in the central zone (P < 0. 01) by the GERD with liver constraint pattern group in the open field test were reduced, and the content of Ghrelin in the serum (P <0. 01), the expressions of Ghrelin positive cells in the gastric tissue, and the expressions of GHSR-la protein in the hypothalamus (P < 0. 01) were increased-Compared with the sham operation group, the contents of GABA in the hypothalamus of rats in the GERD with liver constraint pattern group were reduced (P <0. 05). Conclusion EGDA combined with CUS can successfully establish the GERD with liver constraint pattern models. The expressions of the Ghrelin in the serum and gastric tissue and the GHSR-la protein in the hypothalamus in the rat models may increase while the contents of GABA may decrease. [ABSTRACT FROM AUTHOR]