Immobilization of cellulase enzymes on nano and micro-materials for breakdown of cellulose for biofuel production-a narrative review.

Cellulose is a very abundant polymer that is found in nature. Cellulose has been used as a raw material for production of biofuels for many years. However, there are multiple processing steps that are required so that cellulose can be used as a raw material for biofuel production. One of the most important steps is the breakdown of cellulose into intermediate sugars which can then be a viable substrate for biofuel production. Cellulases are enzymes which play a role in the catalysis of the breakdown of cellulose into glucose. Nanomaterials and micromaterials have been gaining a lot of attention over the past few years for its potential in immobilizing enzymes for industrial procedures. Immobilization of enzymes on these nanomaterials has been observed to be of great value due to the improvement in thermal stability, pH stability, regenerative capacity, increase in activity and the reusability of enzymes. Similarly, there have been multiple reports of cellulase enzymes being immobilized on various nanoparticles. The immobilization of these cellulase enzymes have resulted in very efficient processing and provide a great and economic solution for the processing of cellulose for biofuel production. Hence in this paper, we review and discuss the various advantages and disadvantages of enzymes on various available nanomaterials. • Immobilization of cellulase onto MNPs makes the recovery process easier. • Cellulase immobilized on composite materials has high loading capacity and great reusability. • CNTs are suitable for enzyme immobilization especially for the purpose of biofuel production. • Immobilization on MOF was observed to be an excellent strategy for the improvement in enzyme activity. [ABSTRACT FROM AUTHOR]