Gal和PEI修饰磁性纳米颗粒载体介导的c-Met siRNA构建及其抗肝癌细胞效应观察.

Objective To construct the galactose（Gal） and polyethyleneimine（PEI）-modified magnetic nanocarriers（Gal-PEI-SPIO）-mediated c-Met siRNA and to observe its antihepatocarcinoma effect. Methods Gal-PEI-SPIO nanocarriers were prepared and characterized. Agarose gel electrophoresis was used to detect the combination of carriers and siRNA. The cytotoxicity of the nanocarriers was determined by CCK-8. At the same time， q-PCR and Western blotting were used to detect the silencing efficiency of carriers carrying c-Met siRNA in cells. The effects of Gal-PEI-SPIO/c-Met siRNA nanocomposites on the migration and invasion of liver cancer MHCC-97H cells were detected by Transwell chamber method. Result Agarose gel electrophoresis showed that Gal-PEI-SPIO nanocarriers and siRNA could be closely combined when the SPIO： siRNA mass ratio was 4：1 or more. Simultaneously, when the ratio was more than 4：1，the cell activity decreased rapidly. The qPCR results showed that Gal-PEI-SPIO/siRNA nanocomposites effectively reduced the level of mRNA in MHCC-97H cells, and Western blotting showed that the Gal-PEI-SPIO/c-Met siRNA nanocomposites effectively reduced c-Met mRNA and protein levels （all P<0. 01）. In cell invasion and migration experiments, GalPEI-SPIO/c-Met siRNA nanocomposites significantly reduced the invasion and migration abilities of MHCC-97H cells. Conclusion Gal-PEI-SPIO nanocarriers can bind tightly to siRNA, and can carry c-Met siRNA into hepatocellular carcinoma cells to silence c-Met, thereby inhibiting the invasion and migration of MHCC-97H cells. [ABSTRACT FROM AUTHOR]