A STUDY OF THE EFFICIENCY OF CULTURE MEDIA FOR THE RECOVERY OF LYOPHILIZED PATHOGENIC BACTERIA.

Today, the most appropriate way of long-term storage of most microorganism strains in collections is their lyophilization. Lyophilized bacterial cultures biological properties recovery rate and quality depends on the quality of culture media used for this purpose. The aim of this study was to investigate the effectiveness of culture media for the recovery of lyophilized pathogenic bacteria. This study used strains of Salmonella typhimurium, Pasteurella multocida, Yersinia pseudotuberculosis, and Staphylococcus aureus from the collection of Ukrainian Laboratory of Quality and Safety of Agricultural Products of National University of Life and Environmental Sciences of Ukraine. In order to restore lyophilized bacterial cultures, several variants of nutrient media were used: Meat-Peptone Broth (MPB) culture media (Pharmactive, Ukraine) were used with the addition of 5% horse serum and 0.5% glucose; Meat-Peptone Broth (MPB) (Pharmactive, Ukraine) with the addition of 5% bovine serum and 0.5% glucose; Meat-Peptone Broth (MPB) (Pharmactive, Ukraine) with the addition of 5% serum of cattle and 0.5% of glucose; Brain-hearth infusion broth (BHIB) (HiMedia, India) and Tryptone soya broth (TSB) (HiMedia, India). It was found that in the process of sublimation and cryopreservation of cultures of Salmonella typhimurium, Pasteurella multocida, Yersinia pseudotuberculosis and Staphylococcus aureus, the loss of viable microbial cells was 99.91-99.987%. The concentration of viable microbial cells in lyophilized cultures of Salmonella typhimurium, Pasteurella multocida, Yersinia pseudotuberculosis, Staphylococcus aureus was recorded in the range of 1.4 x 1041.7 x 106 CFU / cm3. During a single passage on different culture media obtained biomass of the studied cultures was in the initial titer x x 109. The highest productivity in reconstituted cultures was obtained using BHIB manufactured by HiMedia, India (concentration of live microbial cells in experimental cultures was recorded in the range of 5.7-6.4 x 109 CFU / cm3). [ABSTRACT FROM AUTHOR]