黄精根际及药用部位内生真菌群落组成和生态功能分析.

To find microbes promoting the biosynthesis of active metabolites in medicinal plants, with Polygonatum sibiricum as materials, the ITS region of fungi form rhizosphere, rhizome and root was sequenced by Illumina MiSeq to analyze the diversity and community composition, and FUNGuild was used to analyze the ecological function categories of endophytes fungi in rhizome. The results were as follows:(1)A total of 1 023 operational taxonomic units(OTUs)were obtained from all samples of P. sibiricum. The numbers of OTU from rhizosphere, rhizome and root fungi were 703, 128 and 141, respectively. The composition of fungal community in three samples was significantly different, and there was specific fungi Chytridiomycota in root.(2)There was 41 OTU in common of three samples, and Ascomycota accounted for the highest proportion(58.15%).(3)In ecological functional, endophytic fungi of rhizome in P. sibiricum contained six functions groups, undefined saprotroph, mycoparasites, plant pathogen, animal pathogen, arbuscular mycorrhizal fungi, lichenized fungi, and 37 undefined species(34.91%)had no reference information in the FUNGuild database. It is suggested that the dominant endophytic fungi like Setophoma and Neocosmospora in rhizome may be important microbial communities affecting the biosynthesis of the active products, which provides data reference for the exploration of medicinal functional microorganisms in P. sibiricum. [ABSTRACT FROM AUTHOR]