Papilomavirus bovino tipo 2 en un sarcoide equino en Costa Rica.

Objective. To identify by molecular techniques the presence of bovine papillomavirus (BPV) in an equine sarcoid, and to carry out its histological characterization. Materials and methods. Using a sarcoid lesion from a Pinto Americano mare, nucleic acid extraction and identification by molecular techniques was performed. A partial region of the gene corresponding to the L1 segment of papillomavirus was amplified by polymerase chain reaction, then it was sequenced and analyzed using BLAST algorithm. Hematoxylin-eosin staining of the tissue was performed and analyzed under a 100X light microscope. Results. BPV-2 was detected in the equine sarcoid lesion, and the lesion was classified as nodular according to its morphological and histological characteristics. Histopathology showed a fibrotic lesion, with exophytic neoplasia and expansion of the spinous layer. Conclusions. The study established for first time the presence of BPV-2 in an equine sarcoid in Costa Rica using molecular techniques and its association with a nodular-type sarcoid. [ABSTRACT FROM AUTHOR]